Polygon AI

POLYGON AI

New Interface, New Features, Same Analysis You Trust

Polygon AI is the latest in Rewire’s mission to bring powerful and adaptable artificial intelligence to biomedical research. With new features like Batch Image Processing and all-new Segmentation analysis capabilities, Polygon is better, easier-to-use, and now free for everyone.

Take the guess work out of image analysis and simplify your detection and quantification by downloading Polygon today.

MULTI-LABEL ANALYSIS

Polygon AI offers both single and multi-label analysis with advanced colocalization features and a pre-loaded detection model library.

EASY-TO-USE INTERFACE

Polygon’s interface harnesses the power of our AI engine, Sightologst.ai, to deliver the accuracy you expect in a smooth and responsive platform.

DATA EXPORT

Easily export your results to comma-seperated-values (.csv) files that can be integrated into Excel or other statistics packages. 

CUSTOM DETECTION MODELS

With the power of Sightologist.ai, you can train custom detection models to fit any need with only a few steps, all using Polygon’s interface – no coding required.

Better, Faster, More Accurate

Polygon AI (formerly Pipsqueak Pro) is published and proven to increase the accuracy and reliability of your data while reducing costs.

Reduction in Analysis Cost

Faster than
Manual Detection

Less Data Variability

Finish Your Project Faster, Without Complication

Polygon AI is the only tool you need for image analysis. By utilizing a “human-in-the-loop” process of automatic detection and manual verification, you work alongside our patented artificial intelligence engine, Sightologist.ai, to ensure the best results possible. This collaborative effort is made possible through our line of intuitive features like cloud sync and recommend-a-model, and through the use of our custom model training process.

Cloud Sync and Share

Share your custom AI detection models with others in your lab to cut down on duplicate work and increase the consistency of your results.

Recommend-a-Model

Not sure which pre-loaded detection model works best with your image set? Use the “Recommend-a-Model” feature to let our AI decide for you.

Custom Model Training

Polygon comes pre-loaded with a library of AI detection models ready for use, but if you need something more specific, you can tune these models or create one of your own.

Reliable and Reproducible. Every Single Time.

Manual image analysis can be accurate or precise, but rarely both, and usually only after years of experience. The integration of machine learning into image analysis improves the speed, accuracy, and reproducibility of microscopy quantification across both inter- and intra-rater reliability tests. In other words, AI gets you the same results every single time. This doesn’t just mean that the same work can be done faster, but that more work can be done in the same amount of time.

See for yourself how Polygon AI can improve your image analysis workflows by downloading for free today.

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Polygon By the Numbers

Polygon was first published (as PIPSQUEAK) in 2016 and has since been used by hundreds of researchers to improve the speed, accuracy, and reproducibility of their image analysis.

Peer-REviewed Citations

See the full citations list here

Images Analyzed Per Month

Polygon is completely hardware agnostic, and can be used with any non-proprietary image file types.

Users Around the World

Polygon is used on every continent (except Antarctica, because penguins don’t have microscopes).

Get Started Today

We are confident that the free version of Polygon is powerful enough for most users, but for researchers who are looking for more advanced features and capabilities, we also offer a Premium license.

All purchasing is now handled in-app. Just download the Polygon application to get started!

premium

$975

per year

  • Batch Image Processing
  • 3D Z-Stack Image Processing
  • Individualized Support
  • Unlimited Custom Model Training
  • Unlimited AI Image Processing
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Transitioning from Pipsqueak Pro?

Click below to find out what to expect when you download Polygon.

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Researcher Testimonials

Don’t take our word for it – here’s what some of our users say:

“Critically, [Polygon] generates reliable and sensitive results that can be easily cross-referenced by multiple investigators for consistency and bias. In contrast to manual image quantification, Polygon is capable of analyzing an image in mere minutes versus hours.

In my experience, Polygon is the best tool currently available to investigators working on perineuronal net analysis in the field of neuroscience.”

Yevgeniva

Johns Hopkins University

“Our recent paper (Reinhard et al., 2019, Neurobiology of Learning and Memory) used [Polygon] extensively to analyze perineuronal net intensity in the auditory cortex, hippocampus and amygdala in an autism mouse model. For my trainees to have conducted this work without the semi-automated analysis afforded by your program would have delayed the paper by at least a year.

Polygon has allowed for more consistency between experiments and personnel by setting constant backgrounds and accurately tagging the cells we are specifically interested in.”

Khaleel

University of California, Riverside

Polygon is the gold standard in the field for quantifying the density of these structures. Polygon’s algorithms excel above other techniques by setting a consistent background and selecting only the fluorescent structures that exceed the background and fulfill parameters input by the user. This allows for more consistent analysis not only between experiments, but users as well.”

Kristin

Medical University of South Carolina

“Polygon has become an integral part of my dissertation research, greatly reducing the time needed to conduct image analysis and becoming a staple in the lab that many members use every day.”

Kelsea

Northwestern Univeristy

Download Polygon AI For Free Today!